High-contrast imaging of chlorophyll-containing protein complexes separated by native polyacrylamide gel electrophoresis
نویسندگان
چکیده
Electrophoregrams with bands of green chlorophyll-containing protein complexes separated by native polyacrylamide gel electrophoresis (PAGE) are usually photographed in white light or scanned by a narrow beam of monochromatic light using densitometers. Present CCD cameras allow taking densitograms of a whole gel surface with many samples. However, commercial CCD imaging systems use mostly white or broad-band light scanning for densitometry measurements and are not constructed for the measurements by monochromatic light. In this work we present an application of a home-made two-dimensional CCD imaging system for monochromatic densitometry of chlorophyll-containing proteins separated by PAGE.
منابع مشابه
2-D gel densitometer for high-contrast and selective imaging of chlorophyll-containing protein complexes separated by non-denaturing polyacrylamide gel electrophoresis.
In this work, we present a home-made two-dimensional (2-D) CCD imaging system for the monochromatic densitometry of plane gels and its application to the imaging and densitometry of chlorophyll (Chl)-containing proteins separated by non-denaturing polyacrylamide gel electrophoresis. The monochromatic imaging of separated green bands at the wavelengths corresponding to their absorption band incr...
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